Which pump used in HPLC technique?
Most HPLC pumps are reciprocating pumps. The solvent is drawn into a small chamber (with the solvent check valve open) and pumped out of it (when the column check valve is open) by the back and forth motion of a motor driven piston.
How does the pump work in HPLC?
An HPLC pump is also called a “solvent delivery system.” The purpose of the pump is to maintain a constant flow of mobile phase through the HPLC system. This is accomplished regardless of the back pressure caused by the flow resistance of the HPLC column.
What is dual piston pump?
A reciprocating pump design which produces a pressure differential across the pump on both the in and out stroke of each drive rod. Dual action pumps use two separate pumping chambers per drive rod. On the forward stroke, it pushes fluid out of the first chamber, and draws fluid into the other.
Why are multiple pumps used in HPLC?
Dual-Piston Pumps have two pistons operating in parallel, fully out-of-phase with each other, to produce naturally smooth fluid flow. This is critical for many analytical chromatography applications.
Which pump is not used in HPLC?
Which of the following is not an advantage of Syringe type pumps used in High-pressure liquid chromatography? Explanation: The limitation of Syringe type pump is that it has the limited solvent capacity and is inconvenient when solvents are to be changed.
Why reciprocating pump is used in HPLC?
Dual Piston Reciprocating Pumps Pressure pulses are easily overcome by using dual piston HPLC pumps which incorporate pump heads operating exactly 180° out of phase which results in uninterrupted delivery of mobile phase at constant pressure.
How many pumps are in HPLC?
There are 3 main types of HPLC pumps: 1. reciprocating pump – 90% usage. The solvent is drawn into a small chamber (when the solvent check valve is open) and pumped out of it (when the column check valve is open) by the back and forth motion of a motor-driven piston (Fig 28-6 or R5 Fig 6.9).
Why C18 column is used in HPLC?
C18 is octyldecylsilane and contains 18 carbons bound to the silica. So they have more carbons and a longer carbon chain than C8 (8 carbons) or C4 (4 carbons). Because of the extra carbons, C18 has a larger surface area that the mobile phase has to travel across.
What does HPLC stand for?
High-performance liquid chromatography
High-performance liquid chromatography (or high-pressure liquid chromatography, HPLC) is a chromatographic technique that can separate a mixture of compounds and is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of the mixture.
What do double-acting pumps do?
Double-acting hydraulic cylinders have the ability to pump hydraulic fluid to both sides of the plunger. Connection ports positioned near both ends allow the piston rod to move both forwards and backwards.
How many types of pumps used in HPLC?
There are two common types of HPLC pumps: reciprocating pumps and syringe pumps. Reciprocating pumps are by far the most common pump used and can have single piston or dual pistons.
What is the detector in HPLC?
UV detector is a very commonly used detector for HPLC analysis. During the analysis, sample goes through a clear color-less glass cell, called flow cell. When UV light is irradiated on the flow cell, sample absorbs a part of UV light.